Drag-and-Drop Protein Synthesis: Transcription - zeroBio

Transcription is the first of overall two protein synthesis steps

Since VIP is thought to have growth promotingproperties through VIP receptor activation (,),the treatment of human tumors with a VIP antagonist may lead to theinhibition of tumor growth (). The VIP-receptor antagonist VIPhyb was synthesized as ahybrid peptide of neurotensin and VIP consisting of an N-terminalLys-Pro-Arg-Arg-Pro-Tyr (designed to increase membranepermeability), followed by the C-terminal 22 amino acids of VIP(). This broad spectrum VIPreceptor antagonist inhibited non-small cell lung cancer (), breast cancer (), and colon cancer () growth and . In addition, a further modification of VIPhyb was theaddition of a stearyl group at the N-terminus and the exchange ofthe methionine at position 17 with norleucine. These modificationsresulted in an antagonist with improved affinity (SN)VIPhyb(). (SN)VIPhyb bound the VPAC1receptor with an ∼10-fold greater affinity than VIPhyb and acted asa cytostatic agent in non-small cell lung cancer () and pancreatic cancer () (). Moreover, (SN)VIPhyb enhanced theanti-proliferative effects of chemotherapeutic agents on cancercell lines ().

Includes all the major steps of Protein Synthesis and a quiz at the end

The PrimeScript First Strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of first-strand cDNA from total or poly(A)+ RNA using PrimeScript Reverse Transcriptase. Choose this cDNA synthesis kit when you want to synthesize full-length cDNA with provided primers (select from oligo dT or random 6-mer primers, both included in the kit.) This cDNA synthesis kit is powered by PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity and efficiently synthesizes cDNA. PrimeScript RTase is robust, versatile and well-suited for applications requiring full-length cDNA such as cDNA library construction. This PrimeScript-based cDNA synthesis kit also works well with other cDNA-dependent techniques including RT-PCR, cDNA probe preparation and real-time quantitative RT-PCR (qRT-PCR or RT-qPCR). The PrimeScript First Strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.

Protein synthesis - Biology-Online Dictionary

Teaching Protein Synthesis (Replication, Transcription, and Translation) is a challenge

Delgado M and Ganea D: Vasoactiveintestinal peptide and pituitary adenylate cyclase-activatingpolypeptide inhibit interleukin-12 transcription by regulatingnuclear factor kappaB and Ets activation. J Biol Chem.274:31930–31940. 1999. :